HIV-1 Confirmation assay time comparison

  Typical Western Blot   FLUOROGNOST IFA
  Day Overnight  
Room Temp 10 min 10 min 10 min
Pre-Rock 10 min 10 min  
Incubate 2 hr 18-20 hrs 30 min
Wash 10 min 10 min 10 min
Conj Incubation 1 hr 1 hr 30 min
Wash 10 min 10 min 10 min
Conj 2 Incubation 1 hr na  
Wash 10 min na  
Substrate 15 min 15 min  
Wash 10 min 10 min  
TOTAL TIME 5+ HOURS 20-22 HOURS 90 minutes
STEPS 10 8 5
Sample Volume 30 uL 20 uL 10 uL